Pyser-SGI Limited


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Optical Thin Film Coatings



Pyser-SGI now offer a complete optical thin film coatings service, from single layer to multi layer A/R coatings to dichroic, mirror and band-pass filter coatings. Using Essential Macleod software, a variety of coating processes and Spectrometer instruments we can guarantee the quality of the finished coating.

We can test the spectral response of the finished product in reflection, absorbance and transmission modes from 200 to 3200nm.

Our modern coating plants all have in-process ionic cleaning and thickness monitoring. Our cold vacuum deposition allows us to coat high performance anti-reflective films and many other coatings onto cemented and painted components.

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New Plain Sedgewick Rafter Counting Chamber


For many applications, particularly where larger plankton are being analysed, there is no requirement for a counting grid printed onto the slide, hence the introduction of this slide with a plain glass base. This chamber can be used with either upright or inverted microscopes.

The S53 can be used in conjunction with an eyepiece reticle, such as a Whipple grid (aka Whipple micrometer disc), where detailed counting/sizing is required. Pyser reference for Whipple grid is NE29.

The glass base is 76mm x 38mm x 2mm thick and the Stainless steel frame traps a cell volume of 1.0ml when the cover glass (supplied) is placed over it. The preparation and filling method is exactly the same as the S50 and S52 gridded chambers.

Pattern Description Order code

Sedgewick Rafter Plain Counting Chamber (glass), without grid pattern

Includes 1 cover glass







New You Tube Videos

Pyser now have three You Tube videos to give technical guidance for you and your customers.

Ian Gordon, our Senior Technical Support Specialist, has created these videos to answer all of those little questions that get asked every day.

One video deals with calibration of microscopes using a stage micrometer, the second one shows how to size and fit an eyepiece reticle and the third one shows how to use Sedgewick Rafter counting chambers. These are invaluable tools in the world of microscopy.

Feel free to provide links into these You Tube videos from your websites. Click on the images below to link direct to the videos.


How to Calibrate a Microscope with a Stage Micrometer


How to Size and Fit an Eyepiece Reticle


How to use a Sedgewick Rafter Counting Chamber










BIOS & Photonics West Exhibitions

See us on at BIOS and Photonics West exhibitions in San Francisco

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BIOS Exhibition - January 28th and 29th 2017

Photonics West Exhibition - January 31st to February 2nd 2017